Serum containing ovine IgG2 antibody specific for maedi visna virus envelope glycoprotein mediates antibody dependent cellular cytotoxicity.
作者： Inderpal Singh , Ian McConnell , Robert Dalziel , Barbara A. Blacklaws
DOI: 10.1016/J.VETIMM.2006.06.006
关键词:
摘要: Antibody-dependent cell-mediated cytotoxicity (ADCC) specific for maedi visna virus (MVV) has never been described. The IgG antibody response to MVV is restricted an IgG1 whilst IgG2 seen in persistently infected sheep. To determine whether the isotypic restriction of responsible lack ADCC, ADCC assay was developed using polyclonal serum raised recombinant ENV protein. Sheep immunised with a GST:SUenv fusion protein complete Freund's adjuvant produced which contained and antibodies. activity this compared from Serum sheep mediated reactions no ever serum. may therefore be possible effector isotype against MVV. Failure dependent system vivo contribute persistence MVV-infected macrophages vivo.
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