Comparison of cytotoxic and inflammatory responses of photoluminescent silicon nanoparticles with silicon micron-sized particles in RAW 264.7 macrophages.

摘要: Photoluminescent silicon nanoparticles have a bright and stable fluorescence are promising candidates for bio-imaging, cell staining drug delivery. With increasing development of nanotechnology applications biomedicine, an understanding the potential toxicity is needed to assess safety concerns clinical applications. The objective this study was compare biological responses (SNs, 3 nm diameter) with microparticles (SMs, approximately 100-3000 in cultured murine macrophages (RAW 264.7) using standard protocols assessing cytotoxicity/cell viability inflammatory developed micron-sized particles. SNs SMs were exposed without addition endotoxin lipopolysaccharide (LPS), positive inducer tumor necrosis factor-alpha (TNF-alpha), interleukin 6 (IL-6), nitric oxide (NO). Cytotoxicity assayed dye exclusion MTT assays. Cell supernatants production TNF-alpha, IL-6 NO. at concentrations 20 200 microg ml(-1), respectively, increased cytotoxicity compared controls. induced concentration-related increases TNF-alpha production; contrast, these cytokines shown decrease SNs. NO not by or alone. Fluorescence microscopy demonstrated that associated macrophages, either internalized attached membranes. In conclusion, evaluating differences same material may help improve tests be used biomedical