Effects of particulate and soluble cadmium species on biochemical and functional parameters in cultured murine macrophages

摘要: Cultured murine macrophages (RAW 264.7) were used to evaluate the temporal relationships between cytotoxicity, phagocytosis, tumor necrosis factor-alpha (TNF-alpha), and nitric oxide (NO) production, alterations in expression of stress proteins after exposure cadmium (CdO) or chloride (CdCl(2)), particulate soluble forms cadmium, respectively. Macrophages exposed vitro CdO (25 50 microg) CdCl(2) (30 40 microM) for 2 72 h. Cytotoxicity was not evident until 18 h when 30 microM 25 microg CdO, but occurred as early 12 CdO. Relative untreated controls, phagocytic activity decreased progressively from 24 both cadmium. TNF-alpha levels increased 2- 3-fold 4 remained elevated CdCl(2), by 18-24 at CdCl(2). alone did induce NO; however, species reduced lipopolysaccharide (LPS)-stimulated NO production a dose-dependent manner. Enhanced de novo synthesis 70- 90-kD heat shock, stress, observed 8 CdO; these returned control Stress protein enhanced prior coincided with decrease capacity an increase TNF-a levels. The data suggest that cultured respond similarly form cell type plays pivotal role inflammatory immune responses.