Inhibition of bacterial adhesion and biofilm formation by a heparinized hydrophilic polymer.

摘要: The adhesion and growth of two pathogenic bacteria (Escherichia coli Staphylococcus aureus) on the surface a heparinized hydrophilic polymer were studied. Heparinized is composed poly(vinyl chloride) grafted with poly(ethylene glycol) monomethacrylate, diethylaminoethyl methacrylate, ionically bound heparin. Poly(vinyl was used as control. Plasma protein pre coated polymers also prepared to evaluate effect proteins bacterial adhesion. Polymer films stored in suspensions under gentle shaking at 37 degrees C for 24 hr. amount adherent cells measured by bioluminescent assay adenosine triphosphate. Their structure observed use scanning electron microscope. These evaluations demonstrated that large biofilm formation found chloride), whereas significant reductions no polymer. Bacterial onto plasma coat investigated, it clear these surfaces dependent upon species absorbed proteins.