Human Respiratory Syncytial Virus Memphis 37 Grown in HEp-2 Cells Causes more Severe Disease in Lambs than Virus Grown in Vero Cells

摘要: Respiratory syncytial virus (RSV) is the most common cause of bronchiolitis in infants and young children. A small percentage these individuals develop severe even fatal disease. To better understand pathogenesis disease therapies unique to less-developed infant immune system, a model needed. The neonatal lamb pulmonary development physiology similar that infants, sheep are susceptible ovine, bovine, or human strains RSV. RSV grown Vero (African green monkey) cells has truncated attachment G glycoprotein as compared HEp-2 cells. We hypothesized would more clinical symptoms pathology. confirm hypothesis, lambs were inoculated simultaneously by two different delivery methods (intranasal nebulized inoculation) with either Vero-grown HEp-2-grown Memphis 37 (M37) strain compare viral infection symptoms. Lambs infected cell-derived intranasal nebulization inoculation had significantly higher levels RNA lungs well greater including both gross histopathologic lesions similarly virus. Thus, our results provide convincing vivo evidence for differences infectivity corroborate previous vitro mechanistic studies demonstrating expression