Microbial community analysis of swine wastewater anaerobic lagoons by next-generation DNA sequencing.

摘要: Anaerobic lagoons are a standard practice for the treatment of swine wastewater. This relies heavily on microbiological processes to reduce concentrated organic material and nutrients. Despite this reliance processes, research has only recently begun identify enumerate myriad complex interactions that occur in microbial ecosystem. To further line study, we utilized next-generation sequencing (NGS) technology gain deeper insight into communities along water column four anaerobic wastewater lagoons. Analysis roughly one million 16S rDNA sequences revealed predominance operational taxonomic units (OTUs) clas- sified as belonging phyla Firmicutes (54.1%) Proteobacteria (15.8%). At family level, 33 bacterial families were found all 12 lagoon sites accounted between 30% 50% each lagoon's OTUs. by nonmetric multidimensional scaling (NMS) TKN, COD, ORP, TSS, DO major environmental variables affecting community structure. Overall, 839 individual genera classified, with 223 An additional 321 identified sole The top 25 approximately 20% OTUs low abundances most suggests present at levels. these results demonstrate have distinct which strongly controlled conditions lagoon. Published Elsevier Ltd.