Spatial and temporal changes in the microbial community in an anaerobic swine waste treatment lagoon.
作者： Kimberly L. Cook , Michael J. Rothrock , Nanh Lovanh , John K. Sorrell , John H. Loughrin
DOI: 10.1016/J.ANAEROBE.2009.06.003
关键词:
摘要: Microorganisms are central to both the beneficial (organic degradation, nutrient removal, biogas production) and detrimental (odor production, pathogen contamination) effects of swine waste storage systems. In this study, quantitative (real-time polymerase chain reaction) qualitative (denaturing gradient gel electrophoresis, cloning, sequence analysis) molecular analyses were used track spatial temporal changes in microbial community slurry from a 0.4 ha anaerobic lagoon. The lagoon, located region western Kentucky which has humid, subtropical environment, was sampled on monthly basis (n=10) over period one year at four different depths (top, 51 cm top, 152 bottom >198 cm). concentration diversity Bacteroides sp. seasonal (up 90% decrease between March June). Hespellia other clostridial species, hand, endemic (concentrations up 1.0x10(7) cells mL(-1) slurry) regardless time or lagoon depth. Results suggest that there while effect depth not as pronounced. Seasonal stored wastes may be (directly indirectly) correlated with malodor emissions lagoons.
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