Interactions of ruthenium(II) compounds with sulfasalazine and N,N′-heterocyclic ligands with proteins

摘要: Abstract Two complexes of Ru(II) possessing the general formula [Ru(ssz) 2 L](PF 6 ) were prepared, in which ssz is sulfasalazine and L either 2,2′-bipyridine ( 1 or 1,10-phenanthroline ). The characterized by elemental conductivity analyses, UV–Vis, vibrational, NMR electrospray ionization mass spectroscopies. geometry around ruthenium(II) distorted octahedral. metal ion bound to carboxylate a bidentate way two heterocyclic nitrogens bpy phen. Spectrofluorimetric measurements indicate that bind BSA with affinity constants ranging from 10 4 5  M −1 . Both compounds are significantly cytotoxic chronic myelogenous leukemia cell line concentration dependent manner. UV-light exposure for 5 min increases cytotoxicity three times ten times. In addition, upon UV light irradiation, able damage bovine serum albumin molecule, at concentrations do not affect dark conditions. It worth note also Abl-SH3 protein, responsible development leukemia. Interactions proteins seem be significant photocytotoxic activity these complexes.