Transbilayer distribution of bromine in fluid bilayers containing a specifically brominated analogue of dioleoylphosphatidylcholine.

摘要: We describe in this paper the transbilayer distribution of bromines specifically halogenated phospholipid 1-oleoyl-2-(9,10-dibromostearoyl)-sn-glycero-3- phosphocholine (OBPC). The was determined by X-ray diffraction oriented multilayers mixtures OBPC and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) at 66% relative humidity general approach Franks et al. (1978) [Nature 276, 530-532]. bromine fluid L alpha phase is described accurately a pair Gaussian functions located 7.97 +/- 0.27 A from center bilayer with l/e half-widths 4.96 0.62 A. find that bilayers are as DOPC an additional centered position double bond conclude excellent structural isomorph for under conditions these experiments. obtained complete fully resolved image halogen label because broad due entirely to thermal disorder not experimental limitations [Wiener, M. C., & White, S. H. (1991a) Biophys. J. 59, 162-173]. observed width indicates virtually all hydrocarbon interior accessible bromines. distance between bromine/double-bond headgroup phosphate one-dimensional Patterson maps found be approximately 12 application distributions quantitative interpretation fluorescence quenching experiments discussed. method self-consistent global analysis data permits use sets different instrumental scale factors developed Appendix.