MiR-143 and rat embryo implantation

摘要: Abstract Background To study the role of miR-143 during embryo implantation in rat. Methods MiR-143 expression rat early pregnancy was detected by Northern blot. The relation between and Lifr predicted confirmed bioinformatics method, dual-luciferase activity assay, Western blot immunohistochemistry. MTS, Edu ranswell chamber assays. Results level on gestation day 5–8 (g.d. 5–8) higher than g.d. 3–4 uteri pregnant mainly localized superficial stroma/primary decidual zone, luminal glandular epithelia. not significantly influenced pseudopregnancy, but activation delayed experimentally induced decidualization promoted expression. Over-expression human endometrial stromal cells (ESCs) inhibited cell proliferation, migration invasion. Knockdown proliferation results recombinant luciferase reporters showed that could bind to 3¢-untranslated region (UTR) leukemia inhibitory factor receptor ( ) inhibit translation. Conclusions Uterine may be involved successful pregnancy, especially process blastocyst through regulating . General significance This have potential provide new insights into understanding function implantation.