An aerosol challenge mouse model for Moraxella catarrhalis

摘要: Abstract A simple, reproducible, and non-invasive mouse pulmonary clearance model for Moraxella catarrhalis via aerosol challenge was established. All of eight tested strains could be inoculated into mice at more than 10 5 colony-forming units (CFU)/lung with a concentration 1×10 9 –6×10 CFU/ml in nebulizer. The number bacteria retained 6 h postchallenge 4 CFU/lung while 24 postchallenge, approximate 3 or less remained the lungs. maximum 100 challenged per exposure. lungs adjusted by bacterial concentration, exposure time, negative pressure. Lung tissue sections revealed that were evenly distributed Passive immunization significantly enhanced homologous strain this model. These data indicate will useful evaluating M. vaccine candidates studying roles immunity against .