Interaction of live and dead Escherichia coli O157:H7 and fluorescent microspheres with lettuce tissue suggests bacterial processes do not mediate adherence.
作者： E.B. Solomon , K.R. Matthews
DOI: 10.1111/J.1472-765X.2005.01816.X
关键词:
摘要: Aims: The goal of this study was to determine whether any specific bacterial processes (biochemical or genetic) cell surface moieties were required for the interaction between Escherichia coli O157:H7 and lettuce plant tissue. Methods Results: Escherichia FluospheresTM (fluorescent polystyrene microspheres) used in experiments investigate interactions with lettuce. as they are a nonbiological material, similar size shape cell, but lack ability respond genetically. Live glutaraldehyde-killed E. attached at levels c. 5·8 log10 cells per cm2 following immersion pieces into suspension containing 8 log10 CFU ml−1. In separate experiment, numbers bacteria associated decreased by 1·5 log cm−2 1-min wash. Exposure times 1 min, 1 h, 6 h had little effect on level attachment FluospheresTM, live killed tissue. Significance: These results indicate that not initial tissue.
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