Methylamine reaction and denaturation-dependent fragmentation of complement component 3. Comparison with alpha2-macroglobulin.
作者： J.B. Howard
DOI: 10.1016/S0021-9258(20)79664-X
关键词:
摘要: Complement protein C-3 can covalently incoporate [14C]methylamine with a stoichiometry of 0.85 +/- 0.11 mol/mol protein. The reactive site is located in the larger, Mr = 135,000 peptide subunit C-3. methylamine incorporated as derivative glutamic acid, viz. gamma-glutamylmethylamide, which was identified by high performance liquid chromatography and low resolution mass spectroscopy. shown to undergo specific, denaturation-dependent fragmentation sodium dodecyl sulfate at 90 degrees C. cleavage results partial conversion fragments 84,000 53,000. completely prevented reaction prior C incubation. incorporation (the glutamyl residue) have been reported for alpha2-macroglobulin (Howard, J.B., Vermeulen, M., Swenson, R. (1980) J. Biol. Chem. 255, 3820-3823). A comparison two proteins suggests that they common site.
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