Differential expression of serum proteins in multiple myeloma

摘要: The exact cause instigating multiple myeloma (MM) has not been fully elucidated, and the disease a median survival of 6 months without any treatment. To identify potential biomarkers MM, serum proteins reflecting alteration in their proteomes were analyzed patients with MM compared healthy controls using two-dimensional electrophoresis (2-DE) matrix-assisted laser desorption/ionization time-of flight mass spectrometry. most notable differentially expressed validated by immunoblotting changes mRNA expression evaluated reverse transcription-quantitative polymerase chain reaction (RT-qPCR). A total 11 protein spots found. levels 7 [Immunoglobulin heavy constant µ; proto-oncogene diffuse B-cell lymphoma (DBL2); 26S protease regulatory subunit 4 (P26s4); albumin; haptoglobin; two unknown isoelectronic point (pI) 6.41 molecular weight 35.4 kDa, pI 8.05 27.4 respectively] downregulated controls. Expression gel actin-related 2/3 complex 1A (ARPC1A); immunoglobulin γ 1; fibrinogen α (FGA) fragment D; zinc finger 70 increased patients. Protein expressions ARPC1A, FGA, P26s4 DBL2 measured an independent cohort 12 10 RT-qPCR analysis demonstrated that ARPC1A only mimicked expression, whereas PSMC1 (encoding P26s4) MCF2 DBL2) did exhibit significant between control samples. These represent putative serological for MM.