Interaction of a live attenuated Salmonella Gallinarum vaccine candidate with chicken bone marrow-derived dendritic cells

摘要: Salmonella enterica serovar Gallinarum (SG) is a Gram-negative intracellular host-adapted pathogen that causes fowl typhoid. Attenuated strains of SG are proven and widely used vaccine candidates because advantages like induction strong humoral cell-mediated immune responses. In the present study, we investigated interaction chicken bone marrow-derived dendritic cells (chBM-DCs) with an attenuated (JOL1355) strain secretes heat-labile enterotoxin B subunit protein previously shown to successfully vaccinate chickens. ChBM-DCs were isolated cultured in presence recombinant GM-CSF IL-4 cytokines. The chBM-DCs infected JOL1355 at multiplicity infection 10. was able invade (DCs); however, survival DCs decreased over time. At 24 h post infection, IL-6, IL-10 IFN-γ transcript levels significantly increased JOL1355-infected compared non-stimulated DCs. Flow cytometry analysis showed proportion producing CD40, CD80, MHC class II cultures control. addition, JOL1355-stimulated could induce significant expression IL-2 co-culture autologous CD4+ T cells. Based on these results, conclude capable internalizing live candidate show signs maturation as evidenced by upregulated costimulatory molecules