Demonstration of a partially cryptic epitope of the major cat allergen Fel d 1: consequences for mAb-based standardization of cat extracts.

摘要: Abstract Background: Antiallergen mAbs that do not recognize clinically important isoforms have been described, raising the question of selection for quantifying major allergens in order to standardize allergenic extracts. This is even more critical if can discriminate between different forms allergen molecules with same amino acid sequence. Objective: We sought demonstrate an anti-Fel d 1 mAb was able two cat independently its sequence and determine relative importance stability both various Methods: Anti-Fel were raised mice characterized. By using these mAbs, a two-site ELISA developed quantify Fel mass units. Results: One shown specifically particular form 1. A this capture form. It then (1) quantitative could vary from one extract another, (2) converted into under certain conditions, (3) unconverted may bear IgE epitopes are specific. Conclusion: Although present study emphasizes issue selecting too specific extracts, it also demonstrates very be interest, especially verify since might clinical implications. (J Allergy Clin Immunol 2000;106:669-76.)