The effect of storage on allergen and microbial agent levels in frozen house dust.

摘要: Background: House dust samples collected for exposure studies are often stored variable time periods until analysis. However, there is currently no information on the effects of storage content biocontaminants. Therefore, associations were analysed between levels mite allergens (Der p 1, Der f 1), cat allergen (Fel d 1) and microbial components (endotoxin, β(13)-glucan) one hand duration at −20°C other hand. Methods: Within framework a study influences INdoor factors Genetics Asthma (INGA), from living room floors June 1995 August 1998 extracted according to standardized protocol. The concentrations Der p 1, Der f 1, Fel d 1 β(13)-glucan determined with specific enzyme immunoassays. Endotoxin was quantified using chromogenic kinetic Limulus amoebocyte lysate (LAL) test. All expressed per gram dust Results: Dust (n = 1236) obtained 655 homes in Hamburg, Hettstedt, Zerbst Bitterfeld. Storage (range 8–298 days) grouped into four categories (≤ 60 d; 61–90 d; 91–120 d; > 120 d). After adjustment city residence season sampling, means ratios comparing 2–4 first category not statistically significant endotoxin β(13 glucan). significantly declined increased times samples. Conclusions: house up 10 months has effect allergen, levels. A potential loss during frozen needs further investigations by repeated measurements identical samples.