Roles of fibrin α- and γ-chain specific cross-linking by FXIIIa in fibrin structure and function
作者： Cédric Duval , Peter Allan , Simon D. A. Connell , Victoria C. Ridger , Helen Philippou
DOI: 10.1160/TH13-10-0855
关键词:
摘要: Factor XIII is responsible for the cross-linking of fibrin γ-chains in early stages clot formation, whilst α-chain occurs at a slower rate. Although γ- and was previously shown to contribute stiffness, role both chains determining structure currently unknown. Therefore, aim this study determine individual α- γ-chain during its effects on structure. We made use recombinant fibrinogen (γQ398N/Q399N/K406R), which does not allow cross-linking. In absence cross-linking, intact D-D interface play potential fibre appearance time, stiffness elasticity. Cross-linking played thickening fibres over decreased lysis rate α2-antiplasmin. also showed that timing appearance, straightening fibres, increasing reducing deformation. time density. Our results show independent specific roles formation
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