BRK/Sik expression in the gastrointestinal tract and in colon tumors

摘要: Clones encoding the breast tumor kinase BRK were isolated from a normal human small intestinal cDNA library that was screened with mouse epithelial-specific tyrosine Sik. Although and Sik share only 80% amino acid sequence identity, Southern blot hybridizations confirmed two proteins are orthologues. mapped to distal chromosome 2, which shows conservation of synteny 20q13.3, location gene. expression examined in gastrointestinal tract, colon cell lines, primary samples. Like Sik, is expressed epithelial cells tract undergoing terminal differentiation. also increased during differentiation Caco-2 adenocarcinoma line. Modest increases detected tumors by RNase protection, situ hybridization, immunohistochemical assays. The appears play role signal transduction its overexpression may be linked development variety tumors.