More about the inner filter effect: corrections of Stern–Volmer fluorescence quenching constants are necessary at very low optical absorption of the quencher
DOI: 10.1016/S0022-2313(98)00063-5
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摘要: Abstract The steady-state fluorescence quenching of 2,6-bis(diethanolamino)-4,8-dipiperidino-pyrimido [5,4-d]pyrimidine (dipyridamole) by a stable nitroxyl radical 2,2,6,6-tetramethyl-piperidine-N-oxyl (TEMPO) was used to clear up the influence quencher light absorption on values Stern–Volmer constants (KSV). We demonstrate necessity KSV correction absorbance (Aq) already at very low Aq and rather high KSV. Thus, for
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