De novo Transcriptome Analysis of Sinapis alba in Revealing the Glucosinolate and Phytochelatin Pathways

摘要: Sinapis alba is an important condiment crop and can also be used as a phytoremediation plant. Though it has economic agronomic values, sequence data the genetic tools are still rare in this In present study, de novo transcriptome based on transcriptions of leaves, stems roots was assembled for S. first time. The contains 47,972 unigenes with mean length 1,185 nt N50 1,672 nt. Among these unigenes, 46,535 (97%) were annotated by at least one following databases: NCBI non-redundant (Nr), Swiss-Prot, Kyoto Encyclopedia Genes Genomes (KEGG) pathway, Gene Ontology (GO), Clusters Orthologous Groups proteins (COGs). tissue expression pattern profiles revealed that 3,489, 1,361 8,482 predominantly expressed alba, respectively. leaf enriched photosynthesis- carbon fixation-related pathways. stem not only pathways related to sugar, ether lipid amino acid metabolisms but plant hormone signal transduction circadian rhythm pathways, while root-dominant genes lignin cellulose syntheses, involved plant-pathogen interactions, potentially responsible heavy metal chelating detoxification. Based transcriptome, 14,727 simple repeats (SSRs) identified, 12,830 pairs primers developed 2,522 SSR-containing unigenes. Additionally, glucosinolate (GSL) phytochelatin metabolic which give characteristic flavor tolerance plant, intensively analyzed. aliphatic GSLs pathway roots. absence tissues due shutdown BCAT4, MAM1 CYP79F1 expressions. Glutathione extensively converted into roots, actively oxidized form indicating different mechanisms two tissues. This will benefit basic research molecular breeding useful molecular-assisted transfer beneficial traits other crops.