Imbalance of Molecular Module of TINCR-miR-761 Promotes the Metastatic Potential of Early Triple Negative Breast Cancer and Partially Offsets the Anti-Tumor Activity of Luteolin

摘要: Background Triple negative breast cancer (TNBC) poses a great threat to patient prognosis. LncRNA-miRNA is molecular module formed by long non-coding RNA (LncRNA) and microRNA (miRNA) that mediates the metastatic potential of tumours such as TNBC, luteolin (LU) natural compound with anti-TNBC activity. Objective We aim explore regulatory mechanism terminal differentiation-induced (TINCR)-miR-761 in early well its influence on anti-tumor activity LU. Methods The serum was collected from TNBC patients stage detect expression TINCR miR-761 using RT-PCR. Transwell method applied for determination cell migration invasion, Western blot epithelial-mesenchymal transition (EMT), flow cytometry (FCM) apoptosis, dual luciferase reporter pull-down experiment verification targeted relationship between miR-761. Results Both were up-regulated area under curve (AUC) two distinguishing BC not less than 0.850. In function tests, down-regulation or notably suppressed potentials (cell migration, invasion EMT) cells remarkably inhibited, while up-regulation promoted potentials. also confirmed acts sponge miR-761, has positive regulation it. Besides, LU can significantly down-regulate partially offset when they abnormally up-regulated, which mainly reflected decrease anti-proliferation pro-apoptotic ability against TNBC. Conclusion There an imbalance TINCR-miR-761 may be new therapeutic target