Chromosomal heterogeneity of Helicobacter pylori isolates by pulsed-field gel electrophoresis
作者： Shigeto Takami , Tomoko Hayashi , Yasushi Tonokatsu , Takashi Shimoyama , Toshihide Tamura
DOI: 10.1016/S0934-8840(11)80947-3
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摘要: Summary The genome of 24 Helicobacter pylori ( H. ) isolates was analyzed by restriction endonuclease digestion and pulsed-field gel electrophoresis (PFGE). On PFGE profiles Not I, 12 among yielded four to nine fragments which differed them. other could not be digested with Nod. total size calculated from individual ranged 1.04 × 10 3 1.80 kb. This remarkable uneveness in the is unusual if considered a single species. Therefore, extreme diversity strains, had been as homogeneous according conventional biochemical criteria, led us reexamine species for their genomic homology. Furthermore, we used genetic heterogeneity marker identify specified strains clinical isolates.
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