Development and clinical application of an immunoassay using intact Helicobacter pylori attached to a solid phase as an antigen

摘要: Abstract Objective: At present, H. pylori homogenates, extracts and recombinant proteins are used as antigens in immunoassays, but significant false positive negative results obtained. We attempted to develop an ELISA system using immobilized whole intact cells a solid phase antigen (WIC-ELISA) which specifically recognizes surface antigens. Methods: Intact were on plates by centrifugation (150 g for 10 min). HRP-labeled antihuman IgG was the second antibody. pylori-infections investigated with WIC-ELISA conventional method pylori-extracts 117 patients. Results: showed better characteristics than regards sensitivity (98.9 vs. 90.4%), specificity (95.7 95.7%), predictive value 98.8%), 71.0%) accuracy (98.3 91.5%). Conclusions: is useful quantification of antibodies against clinical screening test.