Oral Vaccination Against Helicobacter pylori with Recombinant Cholera Toxin B-Subunit

摘要: Background.  The innocuous pure recombinant cholera toxin B-subunit (rCTB) is very attractive as a strong adjuvant for host immunization, but little known about rCTB's gastric mucosal immunoadjuvanticity against Helicobacter pylori. of rCTB H. pylori was tested. Material and methods.  Mice were immunized with sonicated H. pylori orally or intranasally sacrificed on day 42 after immunization. Passive cutaneous anaphylaxis (PCA) test performed to evaluate IgE-mediated serum from mice which H. pylori-antigen had been administered. Immunoglobulin titer specific in serum, lavation the gastrointestinal tracts feces examined. Gastritis vaccinated challenge assessed scoring defined grading inflammation. proliferation immunization investigated by counting colony forming units (CFU) per gram stomach tissue. Results.  PCA exhibited no reactions pylori-antigen administered intranasally. Oral nasal coadministrations significantly raised systemic immunities suppressed mucosa. score gastritis higher than that nasally due postimmunization gastritis. Only oral administration compared intranasal without rCTB. Conclusions.  present study indicated has immunoadjuvanticities vaccination might additively support antibiotic eradication.