Structure-Activity Studies of Surface Immobilized Cytochrome c Films on Indium Tin Oxide: Solution Adsorbed and Microcontact Printed Films

摘要: The goal of this research project is to understand how different methods protein immobilization affect the function and orientation a redox active (cytochrome c) on an electrode surface. Cytochrome c (cyt widely thought adsorb onto negatively charged surfaces with heme pocket close in narrow distribution that facilitates rapid electron transfer; however, direct relationship between transfer properties has not been experimentally proven. Establishing structure-function relationships crucial development molecular devices based immobilized proteins (e.g. biosensors). Differences structure activity two types films have studied by comparing their surface coverage, distributions electrochemical behavior indium tin oxide (ITO) Films were formed printing cyt ITO using microcontact (μCP) adsorption out solution. A combination surface-sensitive spectroscopic techniques was used probe prosthetic group protein, extension, as whole. Attenuated total reflectance (ATR) spectroscopy measure coverage second order parameter glass ITO. depended substrate method deposition. Total internal fluorescence (TIRF) fourth these angle absorption emission dipoles Zinc substituted heme. Using parameters,