Comparative immunogenicity of conjugates composed of the Staphylococcus aureus type 8 capsular polysaccharide bound to carrier proteins by adipic acid dihydrazide or N-succinimidyl-3-(2-pyridyldithio)propionate.
作者： A Fattom , J Shiloach , D Bryla , D Fitzgerald , I Pastan
DOI: 10.1128/IAI.60.2.584-589.1992
关键词:
摘要: Staphylococcus aureus type 8 capsular polysaccharide (CP) was conjugated either to diphtheria toxoid or Pseudomonas aeruginosa recombinant exoprotein A by using adipic acid dihydrazide (ADH) N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP) as the joining reagent. The polysaccharide/protein ratios of these two pairs conjugates were similar. synthetic schemes bound linker carboxyls CP carbodiimide-mediated condensation. ADH protein, whereas SPDP reacted with amino groups protein. Intermolecular linking carrier caused carbodiimide during conjugation reaction derivative, probably accounts for larger size formed compared those SPDP. Both synthesized elicited higher levels antibodies, especially after first immunization, than did prepared Similar antibodies both conjugates. Higher conjugate one ADH. basis differences in immunogenicities S. is discussed.
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