Relationships Between Decorin and Biglycan, Structure and Tendon Mechanics Using Mutant Mouse Models

作者: LeAnn M. Dourte

DOI:

关键词:

摘要: Tendons have a complex mechanical behavior that depends on their composition and structure. Understanding structure-function relationships may elucidate important differences in the functional behaviors of specific tendons guide targeted treatment modalities tissue engineered constructs. Specifically, interactions small leucine-rich proteoglycans (SLRPs) with collagen fibrils, association water role fibrillogenesis suggest SLRPs play an tendon mechanics. Some studies assessed response tendon, but between sophisticated mechanics, assembly not been well characterized. Therefore, aim this study was to evaluate structure focus decorin biglycan, two Class I SLRPs. Utilizing homozygous null heterozygous mutant genotype mouse models, amount were varied allow for "dose" A statistical model used explore coordinated roles measured matrix molecules better understand account compensation often seen models. In biglycan mice, no changes any elastic tensile or compressive properties compared wild type. However, viscoelastic altered heterozygotes nulls heterozygotes. Compensatory increases expression other noted genotypes. Changes also found total content structure, although characteristics could completely explain measured. These results viscoelasticity. Finally, multiple regression determine compositional structural components predict properties. Challenges type size discussed. Complex present all models demonstrate importance considering amounts when examining relationships. Degree Type Dissertation Name Doctor Philosophy (PhD) Graduate Group Bioengineering First Advisor Louis J. Soslowsky

参考文章(164)
Y. Ezura, David E. Birk, M. Favata, G. Zhang, S. Chakravarti, L. J. Soslowsky, B. B. Young, Y. Ezura, Development of tendon structure and function: regulation of collagen fibrillogenesis Journal of Musculoskeletal & Neuronal Interactions. ,vol. 5, pp. 5- 21 ,(2005)
M Benjamin, J R Ralphs, Tendons and ligaments--an overview. Histology and Histopathology. ,vol. 12, pp. 1135- 1144 ,(1997)
J Halper, J H Yoon, Tendon proteoglycans: biochemistry and function. Journal of Musculoskeletal & Neuronal Interactions. ,vol. 5, pp. 22- 34 ,(2005)
J E Scott, Proteoglycan-fibrillar collagen interactions Biochemical Journal. ,vol. 252, pp. 313- 323 ,(1988) , 10.1042/BJ2520313
William E. Garrett, Donald T. Kirkendall, Kevin P. Speer, Principles and Practice of Orthopaedic Sports Medicine ,(2000)
László G. Józsa, Pekka Kannus, Human Tendons: Anatomy, Physiology, and Pathology ,(1997)
Benno Nigg, Brian MacIntosh, Joachim Mester, Biomechanics and Biology of Movement ,(2000)
Robert E. Neuman, Milan A. Logan, The determination of hydroxyproline. Journal of Biological Chemistry. ,vol. 184, pp. 299- 306 ,(1950) , 10.1016/S0021-9258(19)51149-8