Microdissection-based allelotyping discriminates de novo tumor from intrahepatic spread in hepatocellular carcinoma

摘要: A total of 103 cases hepatocellular carcinoma (HCC) arising in native livers discovered at the time transplantation underwent allelic loss analysis. HCC mutational allelotyping targeted 10 genomic loci (1p, 3p, 5q, 7q, 8q, 9p, 10q, 17p, 17q, 18q) using 18 polymorphic microsatellite markers situated proximity to known tumor suppressor genes associated with human carcinogenesis. Gene analysis was performed on microdissected tissue samples removed from 4-μm thick histologic sections specific topographic sites selected basis representative cellular characteristics. Microdissection targets included largest nodule 2 locations as well up 3 additional nodules each case. genotyping characteristics including profile and cumulative fractional (FAL) were correlated clinical pathologic features. Individual showed patterns change: (1) essentially concordant profiles consistent intrahepatic spread tumor, or (2) discordant independent primary cancer formation. In 15 56 (27%) which a multinodular, bilobar form (T4), sufficient discordance enabled more accurate T-stage classification better prediction recurrence-free survival. conclusion, microdissection is an effective objective means distinguish between de novo formation versus improve current methods for aggressiveness survival after liver transplantation. (Hepatology 2003;37:871-879.)