Microdissection-Based p53 Genotyping: Concepts for Molecular Testing.

摘要: p53 is the most commonly altered tumor-suppressor gene in humans, involved development and progression of many diverse forms human cancer. Although much remains to be learned about biology this important growth regulatory gene, sufficient experience has been accumulated with respect occurrence pattern mutational change justify molecular diagnostic testing for specific objectives. The authors outline concepts particular emphasis solid tumor diagnostics. This article focuses on microdissection-based fixed tissue analysis, introducing new considerations related quality control appropriate type methodology. Given rapidly evolving nature genetics, suggestions provided here should viewed as flexible. Nevertheless, are intended serve a model other oncogene/tumor suppressor-gene assays developed overall purpose establishing informative integrated histopathologic/genetic complement standard microscopic analysis.