Molecular analysis of RNA1 and RNA2 sequences from a betanodavirus isolated from giant grouper (Epinephelus lanceolatus) in Australia
作者： Kalpana Agnihotri , Brad Pease , Roger Chong
DOI: 10.1016/J.VIREP.2016.05.001
关键词:
摘要: Betanodavirus infections have a significant impact through direct losses and trade restrictions for aquaculture sectors in Australia. The giant grouper, Epinephelus lanceolatus, is high-value, fast-growing species with potential. With subacute to chronic mortalities reported from commercial facility northern Queensland, the viral nervous necrosis affected fish was confirmed using RT-qPCR followed by virus isolation SSN-1 cell line. RNA1 RNA2 segments were sequenced nucleotide sequences compared betanodavirus GenBank. Phylogenetic analysis revealed that both these clustered representing red spotted grouper genotype showed high sequence identity affecting other species. This first report confirming infection E. lanceolatus Australia successful of culture system, nearly full length sequences.
daf.qld.gov.au
core.ac.uk参考文章(19)
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