Regenerative Proliferation in Organ Cultures of the Avian Cochlea: Identification of the Initial Progenitors and Determination of the Latency of the Proliferative Response

摘要: Sensory hair cells in the cochleae of birds are regenerated after death preexisting caused by acoustic overstimulation or administration ototoxic drugs. Regeneration involves renewed proliferation an epithelium that is otherwise mitotically quiescent. To determine identity first proliferate response to and measure latency this proliferative response, we have studied hair-cell regeneration organ culture. Cochleae from hatchling chicks were placed culture, killed individually a laser microbeam. The culture medium was then replaced with contained labeled DNA precursor. treated incubated labeling media for different time periods before being fixed processed visualization proliferating cells. initiate replication supporting within cochlear sensory epithelium. All located 200 μm lesions. These entered S-phase ∼16 hr results indicate precursors suggest regenerative triggered signals act locally damaged