Leukemia diagnosis and testing of complement-fixing antibodies for bone marrow purging in acute lymphoid leukemia

摘要: In this paper a microplate method is described for diagnosing acute leukemia and investigating the reactivity of monoclonal antibodies (MoAbs) against membrane antigens in combination with rabbit or murine to nuclear terminal transferase (TdT). The speed facilitates investigation fresh leukemic cells from individual patients assesses cytolytic efficacy relevant MoAbs presence complement (C'). Lymphoblasts (TdT+) are mixed equal proportions known numbers “inert” cells, eg, RBC nonleukemic bone marrow (BM). Following incubation C' ratio residual TdT+ inert determined on cytospin preparations. Initially, percentages counted unit volume 5,000 followed by scanning greater than 2 X 10(4) entire slides. With more 4 log cytoreduction TdT + detected. also applicable studying cytolysis malignant B using mostly lg expression rather identification cells. Ten representative selected group 100 reported. some cases no identifiable achieved single C'-fixing MoAb: anti-CD10 (RFAL3) common lymphoid (ALL) anti-CD7 (RFT2) T cell ALL (T-ALL). Other require cocktails anti-CD10, anti-CD19, anti-CD24 anti-CD8 T-ALL. T-ALL few remain that exhibit features normal BM (CD7-, HLA-DR+). This particularly noticeable when studied partial remission if used as source methods here contribute establishing range (ie, IgM class) techniques efficient purging comparing “clean-up,” remission, ALL, T-ALL, malignancies.