The use of correction factors in the determination of mutant frequencies in populations of human diploid skin fibroblasts
作者： A.A. Van Zeeland , J.W.I.M. Simons
DOI: 10.1016/0027-5107(76)90268-2
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摘要: Abstract The need and validity for using a number of correction factors in the determination mutant frequencies human diploid skin fibroblasts was investigated. Resistance to purine analogue 8-azaguanine used as selective system. It appears that, under conditions used, there no effect cell density on cloning efficiency. Therefore observed be corrected reliability Lesch-Nyhan cells prototype estimation efficiency recovery investigated found hold true. relation between density, which reflects degree inter-clone metabolic cooperation, measured. use this relationship enhances accuracy estimated frequencies. With these mutation rate 5.7 · 10 −6 per generation. influence intra-clone cooperation determined small if present.
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