Quantitative High‐Performance Liquid Chromatographic Determination of Δ4‐3 Ketonic Free Steroids in Human Plasma and Amniotic Fluid
作者： Yusuke Sagara , Satoshi Yamanaka , Yoshihiko Takeda , Hisao Tsuruta
DOI: 10.1111/J.1447-0756.1982.TB00553.X
关键词:
摘要: Summary A high-performance liquid chromatographic method is described for the measurement and identification of eight steroids possessing Δ4-3 ketonic compounds in human specimens. The (Cortisone, Cortisol, Corticosterone, DOC, Δ4-Androstendione, Testosterone, 17-Hydroxyprogesterone Progesterone) were separated with a methanol-THF-water system on an IRICA RP-18 column. Effluents detected by UV absorption at 245 nm. Quantitative analysis was performed peak heights, which proportional to amount each substance. This rapid, highly sensitive, easy perform reproducible.
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