作者: P.G. Satyaswaroop , E.Lopez de la Osa , E. Gurpide
DOI: 10.1016/0039-128X(77)90144-1
关键词: Absorbance 、 High pressure 、 Chemistry 、 Resolution (mass spectrometry) 、 Elution 、 Chromatography
摘要: Abstract High Pressure liquid chromatographic procedures for the resolution of mixtures C18 and C19 steroids biologic importance are described. On-line monitoring absorbance refractive index eluate was found to be suitable quantitative measurement amounts eluted steroids. Using a high sensitivity detector, reliable measurements as little 5 ng Δ4−3 keto were possible.