Separation of steroidal estrogens and their major unconjugated metabolites by high performance liquid chromatography.
作者： R.F. Aten , A.J. Eisenfeld , N.J. Maclusky , R.B. Hochberg
DOI: 10.1016/0022-4731(82)90058-9
关键词:
摘要: Abstract A high performance liquid Chromatographic method is described for the rapid, non-destructive separation of a number physiologically important steroidal estrogens, including labile catechol estrogens. This procedure uses “Diol” column and gradient elution to separate in single run, estrogens ranging from 2-methoxy estrone. one least polar C 18 steroids, estriol, most polar. Simpler, isocratic conditions, are provided similar polarity. semipreparative composition was used purification samples containing 25 50 mg individual steroids.
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sci-hub.se 参考文章(14)
Kazutake Shimada, Touichi Tanaka, Toshio Nambara, Studies on steroids Journal of Chromatography A. ,vol. 178, pp. 350- 354 ,(1979) , 10.1016/S0021-9673(00)89730-7
J. Fishman, C. P. Martucci, New Concepts of Estrogenic Activity: the Role of Metabolites in the Expression of Hormone Action The Menopause and Postmenopause. pp. 43- 52 ,(1980) , 10.1007/978-94-011-7230-1_5
Pentti K. Siiteri, [42] A universal chromatographic system for the separation of steroid hormones and their metabolites Methods in Enzymology. ,vol. 36, pp. 485- 489 ,(1975) , 10.1016/S0076-6879(75)36045-X
M. Schöneshöfer, H.J. Dulce, Comparison of different high-performance liquid chromatographic systems for the purification of adrenal and gonadal steroids prior to immunoassay Journal of Chromatography B: Biomedical Sciences and Applications. ,vol. 164, pp. 17- 28 ,(1979) , 10.1016/S0378-4347(00)81567-4
P.G. Satyaswaroop, E.Lopez de la Osa, E. Gurpide, High pressure liquid chromatograph ic separation of C18 AND C19 steroids Steroids. ,vol. 30, pp. 139- 145 ,(1977) , 10.1016/0039-128X(77)90144-1
H Gelbke, A chemical method for the quantitative determination of 2-hydroxyestrone in human urine Steroids. ,vol. 21, pp. 665- 687 ,(1973) , 10.1016/0039-128X(73)90135-9
Mary C. Williams, Joseph W. Goldzieher, Chromatographic patterns of urinary ethynyl estrogen metabolites in various populations Steroids. ,vol. 36, pp. 255- 282 ,(1980) , 10.1016/0039-128X(80)90001-X
P.H. Jellinck, B.J. Brown, A simple enzymatic method for the synthesis of 2-hydroxy[4-14C] estradiol. Steroids. ,vol. 17, pp. 133- 140 ,(1971) , 10.1016/S0039-128X(71)80120-4
H.P. Gelbke, R. Knuppen, A new method for preventing oxidative decomposition of catechol estrogens during chromatography Journal of Chromatography A. ,vol. 72, pp. 465- 471 ,(1972) , 10.1016/S0021-9673(01)91900-4
George R. Merriam, Neil J. MacLusky, Leslie A. Johnson, Frederick Naftolin, 2-Hydroxyestradiol-17α and 4-hydroxyestradiol-17α, catechol estrogfn analogs with reduced estrogen receptor affinity Steroids. ,vol. 36, pp. 13- 20 ,(1980) , 10.1016/0039-128X(80)90063-X