A counterintuitive method for slowing down an analyte in a nanopore and its application to polymer sequencing

摘要: Abstract A major obstacle in nanopore-based polymer sequencing and analysis is the high speed of translocation an analyte (nucleotide, DNA, amino acid (AA), peptide) through pore; it currently exceeds available detector bandwidth. Except for one method that uses enzyme motor to sequence attempts resolve problem satisfactorily have been unsuccessful. Here a counterintuitive based on reversing pore voltage and/or increasing mobility some analytes described. simplified Fokker-Planck model shows increases times 10s ms single nucleotides AAs. More realistic simulations show bi-level positive-negative profile can trap inside ∼1 thereby enable detection four 20 proteinogenic AAs with low While also reduces ionic current, other methods, such as optical, do not depend current blockades detect analyte, take advantage slowdown given here. This potentially viable solution has prevented methods from realizing their full potential.