Regulation of cellular phenotype and expression of polyomavirus middle T antigen in rat fibroblasts.

摘要: Abstract Polyoma middle T antigen (mT) was expressed in rat F-111 cells under control of the dexamethasone-regulatable mouse mammary tumor virus promoter. Graded phenotypic responses to levels mT induction by hormone were seen, with morphological transformation, focus formation, and anchorage-independent growth requiring increasing expression. The ability different clones form tumors reflected their maximum level mT-associated kinase grow soft agar. Expression transformation parameters tumorigenicity correlates phosphorylated pp60c-src immune complexes not total amount determined metabolic labeling. We suggest that cellular factors regulate activity forming a kinase-active fraction molecules controls transformed state.