SIMULTANEOUS IMAGING OF INDIVIDUAL MOLECULES ALIGNED BOTH PARALLEL AND PERPENDICULAR TO THE OPTIC AXIS

摘要: We report the first room temperature observation of an emission pattern from a single fluorescent molecule signifying that dipole is aligned along optic $(z)$ axis microscopic imaging system. This technique takes advantage $x$, $y$, and $z$-polarized evanescent fields generated in total internal reflection usually nettlesome aberrations commonly encountered when biological samples with high numerical aperture oil-immersion objectives. For both $z$-oriented transverse-oriented individual molecules carbocyanine dye ${\mathrm{DiIC}}_{18}$ embedded polymethyl-methacrylate (PMMA), calculated images accurately model main features observed patterns.