Mechanically robust cryogels with injectability and bioprinting supportability for adipose tissue engineering.

摘要: Abstract Bioengineered adipose tissues have gained increased interest as a promising alternative to autologous tissue flaps and synthetic fillers for soft augmentation defect reconstruction in clinic. Although many scaffolding materials biofabrication methods been investigated engineering the last decades, there are still challenges recapitulate appropriate microenvironment, maintain volume stability, induce vascularization achieve long-term function integration. In present research, we fabricated cryogels consisting of methacrylated gelatin, hyaluronic acid, 4arm poly(ethylene glycol) acrylate (PEG-4A) by using cryopolymerization. The were repeatedly injectable stretchable, addition PEG-4A improved robustness mechanical properties. supported human progenitor cell (HWA) derived mesenchymal stromal adhesion, proliferation, adipogenic differentiation maturation, regardless PEG-4A. HWA laden facilitated co-culture umbilical vein endothelial cells (HUVEC) capillary-like network formation, which return also promoted adipogenesis. We further combined with 3D bioprinting generate handleable constructs clinically relevant size. enabled deposition multiple bioinks onto cryogels. bioprinted flap-like had an integrated structure without delamination vascularization. Statement Significance Adipose is defects, challenging restoring maintaining shape, achieving this study, robustness, injectability, stretchability maturation cells. Moreover, on top, forming vascularized constructs. This study demonstrates potential implementation generating volume-stable provides strategy fabricate complex regeneration.