Argonaute-1 directs siRNA-mediated transcriptional gene silencing in human cells
作者： Daniel H Kim , Louisa M Villeneuve , Kevin V Morris , John J Rossi
DOI: 10.1038/NSMB1142
关键词:
摘要: Argonaute proteins are the core components of effector complexes that facilitate RNA interference (RNAi). Small interfering RNAs (siRNAs) targeted to promoter regions mediate transcriptional gene silencing (TGS) in human cells through heterochromatin formation. RNAi have yet be implicated mechanism mammalian TGS. Here we describe role Argonaute-1 homolog (AGO1) directing TGS at promoters for immunodeficiency virus-1 coreceptor CCR5 and tumor suppressor RASSF1A. AGO1 associates with polymerase II (RNAPII) is required histone H3 Lys9 dimethylation AGO1, TAR RNA-binding protein-2 (7TRBP2) Polycomb protein EZH2 colocalize siRNA-targeted RASSF1A promoter, implicating These results establish a connection between TRBP2, RNAPII transcription Polycomb-regulated control expression.
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