The Study of DNA Methylation of bax Gene Promoter in Breast and Colorectal Carcinoma Cell Lines.
作者： Naser Jafargholizadeh , Shahrokh Safarian , Ebrahim Azizi , Seyed Jalal Zargar , Mohsen Alipour
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摘要: The Bcl-2 protein family members have known as essential controllers of mitochondrial pathway apoptosis. Bax is a proapoptotic member family, which well to play crucial roles for apoptosis control. bax has been implicated potential tumor suppressor in certain solid tumors such breast and colorectal carcinoma. DNA methylation promoter associated CpG islands common mechanism gene inactivation cells. Methods: Methylation Specific PCR (MSP) used find the profile cancer cell lines. Results: We not detected any kind "CpG hypermethylation" region T47D, MCF7 (as ER positive), MDA-MB-231 MDA-MB-468 negative) carcinoma-derived lines H29 Caco II. Conclusion: It seems that island could main role down-regulation colon cancers.
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Kristel Schorr, Minglin Li, Stanislaw Krajewski, John C. Reed, Priscilla A. Furth, Bcl-2 Gene Family and Related Proteins in Mammary Gland Involution and Breast Cancer Journal of Mammary Gland Biology and Neoplasia. ,vol. 4, pp. 153- 164 ,(1999) , 10.1023/A:1018773123899
Nika N Danial, Stanley J Korsmeyer, Cell Death: Critical Control Points Cell. ,vol. 116, pp. 205- 219 ,(2004) , 10.1016/S0092-8674(04)00046-7
Jingde Zhu, Xuebiao Yao, Use of DNA methylation for cancer detection: Promises and challenges The International Journal of Biochemistry & Cell Biology. ,vol. 41, pp. 147- 154 ,(2009) , 10.1016/J.BIOCEL.2008.09.003
Michael C Wei, Wei-Xing Zong, Emily H-Y Cheng, Tullia Lindsten, Vily Panoutsakopoulou, Andrea J Ross, Kevin A Roth, Grant R MacGregor, Craig B Thompson, Stanley J Korsmeyer, Proapoptotic BAX and BAK: A Requisite Gateway to Mitochondrial Dysfunction and Death Science. ,vol. 292, pp. 727- 730 ,(2001) , 10.1126/SCIENCE.1059108
Isrid Sturm, Sarantos Papadopoulos, Timo Hillebrand, Thomas Benter, Hans-Joachim Lück, Gerhard Wolff, Bernd Dörken, Peter T. Daniel, Impaired BAX protein expression in breast cancer: mutational analysis of the BAX and the p53 gene. International Journal of Cancer. ,vol. 87, pp. 517- 521 ,(2000) , 10.1002/1097-0215(20000815)87:4<517::AID-IJC9>3.0.CO;2-B
J. G. Herman, J. R. Graff, S. Myohanen, B. D. Nelkin, S. B. Baylin, Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands Proceedings of the National Academy of Sciences of the United States of America. ,vol. 93, pp. 9821- 9826 ,(1996) , 10.1073/PNAS.93.18.9821
B. Mohana Kumar, H.-F. Jin, J.-G. Kim, H.-J. Song, Y. Hong, S. Balasubramanian, S.-Y. Choe, G.-J. Rho, DNA methylation levels in porcine fetal fibroblasts induced by an inhibitor of methylation, 5-azacytidine. Cell and Tissue Research. ,vol. 325, pp. 445- 454 ,(2006) , 10.1007/S00441-006-0201-9
Suzanne Hector, Jochen H.M. Prehn, Apoptosis signaling proteins as prognostic biomarkers in colorectal cancer: a review. Biochimica et Biophysica Acta. ,vol. 1795, pp. 117- 129 ,(2009) , 10.1016/J.BBCAN.2008.12.002
R C Bargou, C Wagener, K Bommert, M Y Mapara, P T Daniel, W Arnold, M Dietel, H Guski, A Feller, H D Royer, B Dörken, Overexpression of the death-promoting gene bax-alpha which is downregulated in breast cancer restores sensitivity to different apoptotic stimuli and reduces tumor growth in SCID mice. Journal of Clinical Investigation. ,vol. 97, pp. 2651- 2659 ,(1996) , 10.1172/JCI118715
T Motyl, B Gajkowska, M Gajewska, J Zarzyńska, M Lamparska-Przybysz, Apoptosis and autophagy in mammary gland remodeling and breast cancer chemotherapy. Journal of Physiology and Pharmacology. ,vol. 57, pp. 17- 32 ,(2006)