Silver nanoparticle induced blood-brain barrier inflammation and increased permeability in primary rat brain microvessel endothelial cells.

摘要: The current report examines the interactions of silver nano particles (Ag-NPs) with cerebral microvasculature to identify involvement proinflammatory mediators that can increase blood-brain barrier (BBB) permeability. Primary rat brain microvessel endothelial cells (rBMEC) were isolated from adult Sprague-Dawley rats for an in vitro BBB model. Ag-NPs characterized by transmission electron microscopy (TEM), dynamic light scattering, and laser Doppler velocimetry. cellular accumulation, cytotoxicity (6.25‐50 mg/cm 3 ) potential (interleukin [IL]-1b, IL-2, tumor necrosis factor [TNF] a, prostaglandin E2 [PGE2]) (25, 40, or 80 nm) determined spectrophotometrically, cell proliferation assay (2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2Htetrazolium-5-carboxanilide) ELISA. results show Ag-NPs‐induced cytotoxic responses at lower concentrations 25 40 nm when compared 80-nm Ag-NPs. this study demonstrate both AgNPs size time-dependent profiles, IL-1B preceding TNF PGE2 nm. However, larger (40 induced significant 4 8 h, no observable response. increased fluorescein transport observed clearly indicates size-dependent increases permeability correlated severity immunotoxicity. Together, these data (80 had significantly less effect on rBMEC, whereas smaller effects all end points and/or shorter times. Further, suggests may interact producing a cascade, if left unchecked; events further induce inflammation