Microarray analysis of Long non-coding RNA expression profiles in human gastric cells and tissues with Helicobacter pylori Infection.

摘要: Although Helicobacter pylori (H.pylori) is the dominant gastrointestinal pathogen, genetic and molecular mechanisms underlying H.pylori-related diseases have not been fully elucidated. Long non-coding RNAs (lncRNAs) identified in eukaryotic cells, many of which play important roles regulating biological processes pathogenesis. However, expression changes lncRNAs human infected by H.pylori rarely reported. This study aimed to identify dysregulated gastric epithelial cells tissues with H.pylori. The aberrant profiles mRNAs GES-1 or without infection were explored microarray analysis. LncRNA-mRNA co-expression network was constructed based on Pearson correlation Gene Ontology (GO) KEGG Pathway analyses aberrantly expressed performed related functions pathologic pathways. target validated qRT-PCR confirm data both clinical specimens. Three hundred three 565 as transcripts (≥2 ≤0.5-fold change, P < 0.05) compared controls. showed core lncRNAs/mRNAs might GO indicated that closely inflammation carcinogenesis. QRT-PCR confirmed pattern 8 (n345630, XLOC_004787, n378726, LINC00473, XLOC_005517, LINC00152, XLOC_13370, n408024) cells. Additionally, four down-regulated LINC00473) verified H.pylori-positive samples. Our provided a preliminary exploration H.pylori-infected microarray. These contribute pathological during infection.