Indolyl-3-alkane alpha-hydroxylase compositions from pseudomonas XA

摘要: Compositions containing two species of indolyl-3-alkane alpha-hydroxylase (INDH) are isolated from Pseudomonas XA. An INDH1 composition contains protein subunits having molecular weights 75,000, 34,500 and 32,500 daltons. INDH2 60,000, 44,000 42,000 Molecular determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The compositions have a Specific INDH Activity at least 10 international Units activity per milligram protein, contain less than 1 nanogram endotoxin International Unit Activity. may be immobilized on an insoluble matrix such as silica beads to provide 2.5 gram Immobilized composition. lysing XA cells temperature no more 15° C. form solution INDH, separating the determining concentration solution, if is mg/ml, concentrating ultrafiltration increase within range mg/ml.