Construction and characterization of type 1 non‐fimbriate and non‐adhesive mutants of Salmonella typhimurium

摘要: Mutations in the fimH gene of Salmonella typhimurium result a non-fimbriate, non-adhesive phenotype. This phenotype was shown to be due lack both and fimF expression since disruption by insertion DNA cassette into this determinant results mutants that are complemented plasmids carrying fimF. Deletion mutations within S. carried on recombinant plasmid can used complement mutant, these transformants but fully fimbriate, consistent with role FimH as being necessary for fimbrial adhesin expression. Adherence erythrocytes, HeLa, Hep-2 cells is associated polypeptide, fimbriate strains cannot synthesize non-adhesive. Discrete differences amino acid sequences adhesive type 1 non-hemagglutinating 2 polypeptides were detected, most likely responsible hemagglutinating activity.