Complex Regulation of Membrane-Type Matrix Metalloproteinase Expression and Matrix Metalloproteinase-2 Activation by Concanavalin A in MDA-MB-231 Human Breast Cancer Cells

摘要: Matrix Metalloproteinase-2 (MMP-2) is secreted as a zymogen, the activation of which has been associated with metastatic progression in human breast cancer (HBC). Concanavalin A (Con A) found to induce MMP-2 invasive HBC cell lines. Con effects on expression mRNA for membrane-type matrix metalloproteinase (MT-MMP), newly described surface-associated MMP, showed close temporal correlation induction activation. It surprising that MT-MMP constitutively present uninduced MDA-MB-231 cell, despite lack We have used actinomycin D demonstrate partial requirement de novo gene by cells. Furthermore, this transcriptional response appeared require continued presence its manifestation. The nontranscriptional component manifests rapidly, quite substantial, and persists strongly abrogation both constitutive A-induced MT-MMP. Cycloheximide analyses suggest protein synthesis may be involved rapid transcription-independent response. These studies induces MMP-2-activation part up-regulation but more complicated mode action, involving additional effects, apparently synthesis.