Expression of matrix metalloproteinases in human glioma cell lines in the presence of IL-10.

摘要: Matrix metalloproteinases have been implicated to play a vital role in glioma invasion as they degrade extracellular matrix facilitate the subsequent migration of tumor cells into surrounding brain tissue. The cytokine Interleukin-10 (IL-10) was detected recently glial tumors vivo. Expression specific IL-10 mRNA well blood serum levels patients increased with malignancy suggesting functional progression. Moreover, cell vitro enhanced presence IL-10. We therefore investigated expression (MMPs) stromelysin-1 (MMP-3), 72-kDa collagenase (MMP-2), 92-kDa (MMP-9), matrilysin (MMP-7) and human macrophage metalloelastase (MMP-12). In addition, possible relation between exposure invasiveness these due MMP analyzed. Experiments Matrigel coated Boyden chambers revealed pronounced dose dependent effect on invasiveness. synthetic MMP-inhibitor Marimastat markedly reduced confirming significance MMPs process invasion. Subsequently, level serine protease uPA 7 lines (U373, GaMG, U251, GHE, SNB19, U138 D54) by RT-PCR. all but one line no enhancement detected. Matrilysin U373 only found be upregulated density. present data suggest that related effects invasive properties are not directly mediated an upregulation metalloproteinase expression.