Mutant Aequorea victoria fluorescent proteins having increased cellular fluorescence

摘要: The present invention is directed to mutants of the jellyfish Aequorea victoria green fluorescent protein (GFP) having at least 5 and preferably greater than 20 times specific fluorescence wild type protein. In other embodiments, comprises mutant blue proteins (BFPs) that emit an enhanced fluorescence. also encompasses expression nucleic acids encode a GFP or BFP in wide variety engineered host cells, isolation increased activity. novel allow for significantly more sensitive detection cells possible with its known mutants. Thus, provided herein can be used as reporter molecules detect cell tissue-specific subcellular compartmentalization mutants, chimeric comprising fused regulatory sequence second sequence.